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◎Standard
Type Vectors
These vectors are prepared for the practical use that have the multi cloning site
for your target gene next to the removal cassette.
◎Two
Component Type Vectors
These vectors are custom-made. The pTL7 has the multi cloning site and Sse 8387
I site between RB and LB will be distributed with the ‘hit & run’ cassette.
Please place your target gene at the MCS and insert the ‘hit & run’ cassette
at the Sse 8387 I site.
◎Control
Vectors
These vectors are prepared as control vectors to achieve following objectives
1) to find it possible to distinguish transgenic plants from escape (non-transgenic
plants) visually. 2) to evaluate the promoter for ipt gene in point of the formation
of ESPs. pROL20 is also as a control for rol type MAT vector to verify hairy root
formation.
◎Excision
Monitor Type Vectors
pEXM vector series are constructed for the excision monitor. Since these vectors
have the removal cassette between the promoter and the reporter gene, the gene
introduction is detected in the cells that occurred the removal events. These
are useful to monitor the timing and the location in tissues.
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