A new GST-MAT vector containing both ipt and iaaM/H genes can produce marker- free transgenic tobacco plants with high frequency

Abstract:

Agrobacteriun mediated transformation is highly dependent upon competency of the target plant tissues. It is important to develop the capacity of transformed cells to include cell proliferation and differentiation. The system which results in cell proliferation and differentiation only to transformed cells, is highly desirable for plant transformation. It is reported that a new GST-MAT vector system (MATIMH), in which ipt gene combined with iaaM/H genes was used as the selectable marker gene and GST-II promoter was used as the promoter of the R gene in a site specific recombination system. In tobacco transformation, the combination of the ipt gene and the iaaM/H genes can result in production of both auxin and cytokinin in transformed tissues and induce regeneration of transgenic shoots exhibiting an ipt- shooty phenotype more efficiently than the ipt gene alone. When twenty tobacco leaf discs was transformed with the MATIMH vector, marker free transgenic plants were produced from five (41.6%) out of 12 ipt-shooty lines. These results indicated that the combination of the iaaM/H genes and the ipt gene can more efficiently produce both transgenic plants and marker free transgenic plants.