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Molecular Breeding of Eucalyptus -Analysis of salt stress tolelance of transgenic Eucalyptus camaldulensis |
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Keiko Ymada-Watanabe , Akiyoshi Kawaoka, Etsuko Matsunaga, Kazuya Nanto, Koichi Sugita, Saori Endo, Hiroyasu Ebinuma( Pulp and Paper Research Laboratory, Nippon Paper Industries CO., LTD) ,
Norio Murata (Dept. Regulation Biol., Natl. Inst. for Basic Biol.) |
Tree Biotechnology Symposium 2003 |
| June 7~12,2003 , SWEDEN |
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Abstract:
For the commercialization of transgenic crops and trees, it will become more important to reduce their environmental impact than to increase their economic value. The MAT vector system, which can remove marker genes without sexual crossings and stack valuable genes in sterile clones, is a promising technology for obtaining both regulatory and public approval and to accelerate the commercialization of transgenic trees.
The environmental changes raised by dramatically expanding deserts and salt-containing barrens in many parts of the world caused by climatic changes and human activity. It has negative repercussions on the living conditions and the economic development of the people. Using MAT Vector System, the genetically modified trees tolerance to environmental stress presents a possible new solution for the global problem of soil deterioration with less environmental impact.
Glycinebetaine is one of the compatible solutes that accumulate in the chloroplasts of certain halotolerant plants when these plants are exposed to osmotic stress. The choline oxidase (codA) from Arthrobacter globiformis converts choline into glycinebetain when this bacterium is exposed to the high salt condition. The choline oxidase (codA) is introduced into Eucalyptus camaludulensis with MAT Vector System. We obtained 30 plants transformed with codA gene, besides marker-free plants. The western blot analysis indicated that the codA gene had been integrated and expressed, and that the expressed precursor was processed to the mature protein.
2 transgenic lines were more tolerant to high salt stress than normal plants. The tolerant plants were able to produce roots in medium supplemented with 200 mM NaCl, that almost totally inhibited the rooting of normal plants.
-- Opening possibilities in the prevention of desert expansion by planting salt-resistant eucalyptus -- (Pulp and Paper Reserch Lab. Nippon Paper Industries Co.,LTD.)
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