| Principle of the MAT
Vector® System
The MAT Vector® System is based on
a unique concept that Agrobacterium oncogenes can be used as a selectable marker
to regenerate transgenic plants and select marker-free transgenic plants.
This page is to announce the release of MAT Vector®
System explaining this new plant transformation vector.
Please contuct us
Characteristics of the MAT Vector®
System ref. 1,2
*Autonomous regeneration & visible selection of transgenic plants
*Visible selection of marker-free transgenic plants |
Oncogenes are generally not used as a selectable
marker for plant transformation because the resulting transgenic plants exhibit
a serious abnormal phenotype. The MAT Vector®System is designed to remove
the oncogenes from transgenic plants after transformation by utilizing a yeast
site-specific recombination system, R/RS. ref.
3,4 Two kinds of MAT Vectors are constructed with the oncogenes. (1)
Cytokinin type: the isopentenyl transferase (ipt) gene of A. tumefaciens PO22
is used as a selectable marker. (2) Auxin type: a 7.6 kb EcoR I rol gene fragment
from A. rhizogenes NIAES1724. ref. 5,6
|
Advantages
In current transformation systems, selective
agents (antibiotic, herbicide, etc.) and corresponding resistance genes (selectable
marker genes) are used to select transgenic plants. However, these methods have
three major problems: (1) the selective agents decrease the ability of plant cells
to proliferate and differentiate; (2) the uncertainty of environmental impacts
on many selectable marker genes; and (3) difficulties in repeating a transformation
to increase the number of the desired genes using the same selectable marker.
ref.7 The MAT Vector® System is
designed to overcome these difficulties. ref.8,9
The MAT Vector® System provides an alternate approach to regenerating various
recalcitrant plant species for transformation, through internal manipulation of
the cytokinin-to-auxin ratio, and it also enables the production of marker-free
transgenic plants without sexual crosses and seed production for a pyramid of
multiple genes into a vegetative propagated crop by repeated transformation.
Benefits of MAT Vector®
System
*Improvement of transformation efficiency
*Removal of marker gene for field release
*Multiple introduction of valuable genes
*Pyramid of genes to sterile transgenic trees |
|
|
